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91糖心vlog传媒- 师资队伍- 专任教师- 细胞与生化系

细胞与生化系

李周华

教授，博士生导师

  • 联系方式

    Email: zhli@cnu.edu.cn

  • 招生方向

    硕士研究生招生：071009细胞生物学——03干细胞生物学
    博士研究生招生：071009细胞生物学——干细胞生物学

91糖心vlog传媒

教育及工作经历：

2013-至今 91糖心vlog传媒博士生导师、教授

2010-2013 中国科学院动物研究所副研究员

2010-2010 哈佛大学医学院博士后

2009-2010 新加坡国立大学淡马锡生命科学研究所Research Fellow

2002-2009 新加坡国立大学淡马锡生命科学研究所博士

1999-2002 中国农业大学生物学院硕士

1995-1999 山东农业大学园艺学院学士


研究领域：

干细胞分为胚胎干细胞和成体干细胞。成体干细胞的自我维持和分化在维持成体组织稳态和组织再生中发挥着至关重要的作用。干细胞自我维持和分化失调会导致衰老和癌症等一系列重大疾病的发生(图1)，但调控干细胞自我维持和分化的分子机理尚不明了，因此阐明干细胞自我维持和分化的分子机制对干细胞生物学研究和疾病治疗等领域具有重要的科学意义和应用价值。


1.jpg

图1. 干细胞维持与分化维持组织稳态


本实验室主要以细胞和经典模式生物黑腹果蝇为研究对象，深入研究干细胞微环境（niche）调控成体干细胞(胞囊干细胞、生殖干细胞和肠道干细胞)自我维持和分化及组织稳态的调控机制(图2)。

2.jpg

图2. 本实验室研究的干细胞类型


研究方向包括（但不限于）：

1、干细胞微环境的调控与维持；

2、干细胞维持、增殖与分化的调控机制；

3、组织稳态调控与疾病发生；

4、自噬发生在干细胞维持与分化和癌症中的作用等。


代表性成果 （*通讯作者）：

  1. Ruiyan Kong#, Hang Zhao#, Juan Li, Yankun Ma, Ningfang Li, Lin Shi, and Zhouhua Li*. A regulatory loop of JAK/STAT signaling and its downstream targets represses cell fate conversion and maintains male germline stem cell niche homeostasis. Cell Proliferation, 2024, accepted.

  2. Lin Shi, Hubing Ma, Jinjun Wang, Meifang Ma, Hang Zhao, Zhengran Li, Jian-Hua Wang, Shian Wu, Zizhang Zhou, Meng-qiu Dong, and Zhouhua Li*. An EMC-Hpo-Yki axis maintains intestinal homeostasis under physiological and pathological conditions. Development, 2023, 150(24):dev201958. Research Highlight/亮点文章.

  3. Hang Zhao#, Lin Shi# , Zhengran Li#, Ruiyan Kong, Lemei Jia, Shan Lu, Jian-Hua Wang, Meng-qiu Dong, Xuan Guo, and Zhouhua Li*. Diamond controls epithelial polarity through the dynactin-dynein complex. Traffic, 2023, 24(12):552-563. Cover Story/封面文章.

  4. Ruiyan Kong, Juan Li, Fuli Liu, Yankun Ma, Hang Zhao, Hanfei Zhao, Meifang Ma, and Zhouhua Li*. A feed forward loop between JAK/STAT downstream target p115 and STAT in germline stem cell maintenance in Drosophila adult testis. Stem Cell Reports, 2023, 18(10):1940-1953.

  5. Yueqin Guo#, Ruoyang Hu#, Naikang Li, Nannan Li, Jiangli Wu, Huimin Yu, Jing Tan, Zhouhua Li*, and Shufa Xu*. Autophagy Is Required to Sustain Increased Intestinal Cell Proliferation during Phenotypic Plasticity Changes in Honey Bee (Apis mellifera). International Journal of Molecular Sciences, 2023, 24:1926.

  6. Hang Zhao, Zhengran Li, Ruiyan Kong, Lin Shi, Rui Ma, Xuejing Ren, and Zhouhua Li*. Novel intrinsic factor Yun maintains female germline stem cell fate through Thickveins. Stem Cell Reports, 2022, 17(9):1914-1923.

  7. Hang Zhao, Xuejing Ren, Ruiyan Kong, Lin Shi, Zhengran Li, Runqi Wang, Rui Ma, Huiqing Zhao, Fuli Liu, Henry C Chang, Chun-Hong Chen, and Zhouhua Li*. Auxilin regulates intestinal stem cell proliferation through EGFR. Stem Cell Reports, 2022, 17(5):1120-1137.

  8. Xuejing Ren, Hang Zhao, Lin Shi, Zhengran Li, Ruiyan Kong, Rui Ma, Lemei Jia, Shan Lu, Jian-Hua Wang, Meng-qiu Dong, Yingchun Wang, and Zhouhua Li*. Phosphorylation of Yun is required for intestinal stem cell proliferation and tumorigenesis. Cell Proliferation, 2022, 55(5):e13230.

  9. Fuli Liu#, Hang Zhao#, Ruiyan Kong#, Lin Shi#, Zhengran Li, Rui Ma, Huiqing Zhao, Zhouhua Li*. Derlin-1 and TER94/VCP/p97 are required for intestinal homeostasis. Journal of Genetics and Genomics, 2022, 49(3):195-207.

  10. Hang Zhao, Lin Shi, Zhengran Li, Ruiyan Kong, Xuejing Ren, Rui Ma, Lemei Jia, Meifang Ma, Shan Lu, Ran Xu, Richard Binari, Jian-Hua Wang, Meng-qiu Dong*, Norbert Perrimon*, and Zhouhua Li*. The Yun/Prohibitin complex regulates adult Drosophila intestinal stem cell proliferation through the transcription factor E2F1. Proc Natl Acad Sci USA, 2022. 119 (6) e2111711119.

  11. Ying Yang; Ruiyan Kong, Feng Guang Goh, W Gregory Somers; Gary R Hime; Zhouhua Li, Yu Cai*. dRTEL1 is essential for the maintenance of Drosophila male germline stem cells. PLoS Genetics, 2021. 17(10):e1009834.

  12. Lin Shi, Ruiyan Kong, Zhengran Li, Hang Zhao, Rui Ma, Guang Bai, Jing Li, and Zhouhua Li*. Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation. Biology Open, 2021, 10 (11):bio.058910. Cover Story/封面文章.

  13. Hang Zhao, Lin Shi, Ruiyan Kong, Zhengran Li, Fuli Liu, Huiqing Zhao, Zhouhua Li*. Autophagy induction in tumor surrounding cells promotes tumor growth in adult Drosophila intestines. Developmental Biology, 2021, 476:294-307.

  14. Zhiqiang Bai, Min Wei, Zhouhua Li, Wei Xiao*. Drosophila Uev1a is dually required for Ben-dependent DNA-damage response and fly mobility. Cellular Signaling, 2020, 74:109719.

  15. Ziheng Liu, Ying Yang, Aihong Gu, Jiawen Xu, Ying Mao, Haojie Lu, Weiguo Hu, Qun-Ying Lei, Zhouhua Li, Mingjie Zhang, Yu Cai*, and Wenyu Wen*. Par complex cluster formation mediated by phase separation. Nature Communications, 2020, 11(1):2266.

  16. Min Wei, Lin Shi, Ruiyan Kong, Hang Zhao, and Zhouhua Li*. Heparan sulfate maintains adult midgut homeostasis in Drosophila. Cell Biology International, 2020, 44(3): 905-917.

  17. Hubing Ma#, Huiqing Zhao#, Fuli Liu#, Hang Zhao, Ruiyan Kong, Lin Shi, Min Wei, and Zhouhua Li*. Heparan sulfate negatively regulates intestinal stem cell proliferation in Drosophila adult midgut. Biology Open, 2019, 8(10):bio047126.

  18. Yuanfen Lu, Yuncong Yao*, and Zhouhua Li*. Ectopic Dpp signaling promotes stem cell competition through EGFR signaling in the Drosophila testis. Scientific Reports, 2019, 9(1):6118.

  19. Ran Xu, Jing Li, Hang Zhao, Ruiyan Kong, Min Wei, Lin Shi, Guang Bai, Zhouhua Li*. Self-restrained regulation of stem cell niche activity by niche components in the Drosophila testis. Developmental Biology, 2018, 439(1):42-51.

  20. Zhiqiang Bai, Zhouhua Li, Wei Xiao*. Drosophila Bendless catalyzes K63-linked polyubiquitination and is involved in the response to DNA damage. Mutation Research, 2018, 808:39-47.

  21. Meifang Ma, Hang Zhao, Hanfei Zhao, Richard Binari, Norbert Perrimon, Zhouhua Li*.Wildtype adult stem cells, unlike tumor cells, are resistant to cellular damages in Drosophila. Developmental Biology, 2016, 411(2):207-216.

  22. Zhouhua Li*, Sen Liu, Yu Cai*. EGFR/MAPK signaling regulates the proliferation of Drosophila renal and nephric stem cells. Journal of Genetics & Genomics, 2015, 42(1):9-20.

  23. Yanfen Lu*, Zhouhua Li*. Notch signaling downstream target E(spl)mbeta is dispensable for adult midgut homeostasis in Drosophila. Gene, 2015, 60(1):89-95.

  24. Yanfen Lu*, Zhouhua Li*. No intestinal stem cell regeneration after complete progenitor ablation in Drosophila adult midgut. Journal of Genetics & Genomics, 2015, 42(2):83-86.

  25. Wenyan Ren, Yan Zhang, Min Li , Longfei Wu, Guolun Wang, Gyeong-Hun Baeg, Jia You, Zhouhua Li*, Xinhua Lin*. Windpipe controls Drosophila intestinal homeostasis by regulating JAK/STAT pathway via promoting receptor endocytosis and lysosomal degradation. PLoS Genetics, 2015, 11(4): e1005180.

  26. Xudong Huang, Lai Shi, Jun Cao, Fangfei He, Renling Li, Yan Zhang, Shuang Miao, Longjin Jin, Jia Qu, Zhouhua Li*, Xinhua Lin*. The sterile 20-like kinase Tao controls tissue homeostasis by regulating the Hippo pathway in Drosophila adult midgut. Journal of Genetics & Genomics, 2014, 41(8):429-38.

  27. Zhouhua Li*, Yueqin Guo, Lili Han, Yan Zhang, Lai Shi, Xudong Huang, Xinhua Lin*. Debra-mediated Ci degradation controls tissue homeostasis in Drosophila adult midgut. Stem Cell Reports, 2014, 2(2):135-144.

  28. Yueqin Guo, Zhouhua Li*, Xinhua Lin*. Hs3st-A and Hs3st-B regulate intestinal homeostasis in Drosophila adult midgut. Cellular Signaling, 2014, 26(11):2317-2325.

  29. Yueqin Guo, Ying Feng, Zhouhua Li*, Xinhua Lin*. Drosophila heparan sulfate 3-O sulfotransferase B null mutant is viable and exhibits no defects in Notch signaling. Journal of Genetics & Genomics, 2014, 41(7):369-78.

  30. Zhouhua Li*, Sen Liu, Yu Cai*. Differential Notch activity is required for homeostasis of malpighian tubules in adult Drosophila. Journal of Genetics & Genomics, 2014, 41(12):649-52.

  31. Qinghai Zhang, Yan Zhang, Longfei Wu, Yongfei Yang, Xue Li, Lei Gao, Xiaomeng Hou, Yihui Wu, Guoli Hou, Zhouhua Li*, Xinhua Lin*. dBrms1 acts as a positive regulator of Notch signaling in Drosophila wing. Journal of Genetics & Genomics, 2014, 41(6):317-25.

  32. Jia You, Yan Zhang, Zhouhua Li, Zhefeng Lou, Longjin Jin, Xinhua Lin*. Drosophila Perlecan regulates intestinal stem cell activity via cell-matrix attachment. Stem Cell Reports, 2014, 2(6):761-9.

  33. Zhouhua Li, Yan Zhang, Lili Han, Lai Shi, Xinhua Lin*. Trachea-derived dpp controls adult midgut homeostasis in Drosophila. Developmental Cell, 2013, 24(2):133-143.

  34. Liwei Wang, Zhouhua Li, Yu Cai*. The JAK/STAT pathway positively regulates DPP signaling in the Drosophila germline stem cell niche. Journal of Cell Biology, 2008, 180(4):721-728.

  35. Zhouhua Li, Liwei Wang, Thomas Hays, Yu Cai*. Dynein-mediated apical localization of crumbs transcripts is required for Crumbs activity in epithelial polarity. Journal of Cell Biology, 2008, 180(1):31-38.

研究生招生：

欢迎具有生物学、遗传学、生理学和生物信息学等专业背景、态度端正、强烈希望从事科学研究和认真负责的考生第一志愿报考本实验室。近几年报考本实验室的第一志愿硕士生过线考生数远超本实验室当年招生名额，导致非第一志愿考生很难调剂到本实验室。同时欢迎有志愿报考本实验室研究生的同学来本实验室进行毕业设计或科研预热。


博士后招聘:

本实验室常年招聘有细胞生物学、发育生物学、生理学、生物信息学等研究背景，对以下领域（但不限于）：1）干细胞微环境的调控与维持；2）干细胞生物学和组织稳态调控与疾病发生；3）代谢与干细胞、组织稳态和疾病发生；4）免疫与衰老和疾病发生；5）自噬发生和溶酶体生成与干细胞生物学和疾病发生等研究领域感兴趣的研究人员（近2年或即将取得博士学位）。住房：根据当年实际情况决定（有部分住房可提供）。详细信息请查阅91糖心vlog传媒博士后入站要求。有意者请发送简历（含研究设想（research proposal）和叁名推荐人联系方式）至zhli@cnu.edu.cn。


主持项目：

1、国家自然科学基金面上项目，31271582，气管来源的BMP信号维持果蝇中肠稳态的机理研究，2013/01-2016/12。

2、国家自然科学基金面上项目，31471384，抗增殖蛋白Prohibitin调节果蝇中肠稳态的机理研究，2015/01-2018/12。

3、北京市自然科学基金面上项目，5162004，Stum调节肠道干细胞谱系分化的机理研究，2016/01-2017/12。

4、北京市自然科学基金-市教委联合资助项目，KZ201910028040，全新内源因子Yun调控肠道干细胞增殖和肠道组织稳态的分子机制，2019/01-2021/12。

5、国家自然科学基金面上项目，31972893，内质网膜蛋白复合体调控肠道干细胞增殖和肠道稳态的分子机制研究，2020/01-2023/12。

6、国家自然科学基金委员会重大研究计划“细胞器互作网络及其功能研究”培育项目，92054109，新跨膜因子Angel Face调控质膜向自噬体膜转化的机理研究，2021/01-2023/12。

7、国家自然科学基金面上项目，32370841，m6A修饰调控肠道干细胞增殖和肠道稳态的分子机制研究，2024/01-2027/12。