91糖心vlog传媒

教育经历

2000 年 中国科学院上海生物化学研究所分子生物学博士
1995 年 复旦大学生物化学系生物化学学士

工作经历
2012 年12 月- 91糖心vlog传媒特聘教授
2006 年-2012 年12 月 北京生命科学研究所研究员
2005-2006 年 美国哥伦比亚大学生物系助理研究员
2000-2005 年 美国哥伦比亚大学生物系博士后

研究领域
1. 基因组稳定性
2. 基因表达调控
3. DNA 复制
4. RNA 加工

研究概述
RNA转录和DNA复制是生命过程中必须发生的两个核心生物学事件。然而，我们前期的研究工作意外地揭示，无时无刻进行着的RNA转录可以通过形成R-loop干扰DNA复制叉运行, 进而影响基因组稳定性。维持基因组稳定是生命得以存在和延续的根本。毫无疑问，机体必然相应地发展形成一套有效的应对机制以维护基因组稳定。本实验室的主要研究兴趣在于运用遗传学，分子生物学，生物化学和生物信息学等方法，理解哺乳动物细胞是如何应对在生长和增殖过程中似乎是必然会出现的转录、复制和基因组稳定性,以维护三者间的冲突；并探索这一冲突形成后可能产生的病理后果。希望我们的研究能有助于加深对于肿瘤等重大疾病发病机理的理解，从而为癌症的诊断、治疗和药物的开发提供新的理论基础。

目前实验室正在开展的工作包括：
1. R-loop破坏基因组不稳定性的应对机制
2. 转录在肿瘤发生以及肿瘤细胞染色体不稳定性建立过程中的作用
3. 染色体脆性表达的分子机理
4. 哺乳动物细胞中停顿复制叉的稳定和重新启动的分子机制
5. 人基因组DNA复制起始的时、空调控机制
6. RNA加工过程在癌症发生和发展中的作用
欢迎对上述科学问题感兴趣的同学加入本实验室（接受调剂生）。
 

发表文章 （引文数据来自Google Scholar 查询结果）
1. Yi Wei, Fan Yang, Chuanli Tuju, Jie Liu, Yonggong Zhai and X. Li *, Molecular basis for the expression of chromosome fragility(*corresponding author) (in preparation)
2. B. Liang, P. Peng, S. Chen, L. Lin, D. Cao, J. Yang, H. Li, T. Gui, X. Li* and K. Shen*Characterization and proteomic analysis of ovarian cancer-derived exosomes, Journal of Proteomics, 80 (2013) 171-182 (*corresponding author)
3. W. Jin, Z. Niu, D. Xu and X. Li*, RBM5 promotes exon 4 skipping of AID pre-mRNA by competing with the binding of U2AF65 to the polypyrimidine tract, FEBS Lett 586 (2012) 3852-3857. (*corresponding author)
4. Z. Niu, W. Jin, L. Zhang, X. Li*, Tumor suppressor RBM5 directly interacts with the DExD/H-box protein DHX15 and stimulates its helicase activity, FEBS Lett 586 (2012) 977-983. (*corresponding author) (times cited: 3)
5. Y. Kanehiro, K. Todo, M. Negishi, J. Fukuoka, W. Gan, T. Hikasa, Y. Kaga, M. Takemoto, M. Magari, X. Li, J.L. Manley, H. Ohmori, N. Kanayama, Activation-induced cytidine deaminase (AID)-dependent somatic hypermutation requires a splice isoform of the serine/arginine-rich (SR) protein SRSF1, Proc Natl Acad Sci USA 109 (2012) 1216-1221. (Selected by faculty 1000 of biology)(times cited: 5)
6. W. Gan, Z. Guan, J. Liu, T. Gui, K. Shen, J.L. Manley, X. Li*, R-loop-mediated genomic instability is caused by impairment of replication fork progression, Genes & Development, 25 (2011) 2041-2056. (*corresponding author) (Selected by faculty 1000 of biology) (times cited: 19)
7. X. Li and James L. Manley, 2009, The Role of Alternative Splicing During the Cell Cycle and Programmed Cell Death. In Ralph A. Bradshaw and Edward A. Dennis, editors: Handbook of Cell Signaling 2nd edition, Oxford:Academic Press, , pp.2329-2334. (invited book chapter)
8. X. Li *, Tianhui Niu., and James L Manley. 2007. The RNA binding protein RNPS1 alleviates ASF/SF2 depletion-induced genomic instability. RNA 13(12): 2108-2115 (*corresponding author). (times cited: 11)
9. X. Li and James L Manley, 2006, Co-transcriptional processes and their influence on genome stability. Genes & Development, 2006; 20(14):1838-1847. (times cited: 63)
10. X. Li and James L Manley. 2006. Alternative Splicing and Control of Apoptotic DNA Fragmentation. Cell Cycle 5(12).1286-1288. (times cited: 5)
11. X. Li and James L. Manley, 2005, New talents for an old acquaintance: the SR protein splicing factor ASF/SF2 functions in the maintenance of genome stability. Cell cycle, 4(12): 1706-1708. (times cited: 19)
12. X. Li, Jin Wang and James L Manley, 2005, Loss of splicing factor ASF/SF2 induces G2 cell-cycle arrest and apoptosis, but inhibits internucleosomal DNA fragmentation, Genes & Development, 19(22):2705-2714. (times cited: 76)
13. X. Li and James L Manley, 2005, Inactivation of the SR protein splicing factor ASF/SF2 results in genomic instability. Cell, 122 (3), p365-378. (Selected by faculty 1000 of biology) (times cited: 214)
14. Boliang Li, X. Li et al., 1999, Human acyl-CoA:Cholesterol acyltransferase-1 (ACAT-1) gene organization and evidence that the 4.3-kilobase ACAT-1 mRNA is produced from two different chromosomes, J Biol Chem, 274 (16): p11060-11071.(times cited: 109)
 

获奖情况：
1. 1999 年中国科学院院长特别奖
2. 1999 年中国科学院地奥一等奖